Progress in fluorescent sensors for β-galactosidase
نویسندگان
چکیده
منابع مشابه
Fluorescent Sensors for Biological Applications
Fluorescence is one of the most important analytical methods used in biological studies. In the past decade or two, instrumentation in this field has greatly advanced, and now it is possible to detect single photons or fluorescent molecules [1,2], or break the Abbe diffraction limit to distinguish two points spaced less than 50 nm apart [3]. Concurrently, the development of improved fluorescent...
متن کاملMapping of nuclear localization signals by simultaneous fusion to green fluorescent protein and to beta-galactosidase.
1.Ausubel, F.M., R. Brent, R.E. Kinston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl. 1987. Current Protocols in Molecular Biology. Greene/Wiley Interscience, New York. 2.Chandler, C.S. and F.J. Ballard. 1986. Multiple biotin-containing proteins in 3T3-L1 cells. Biochem. J. 237:123-130. 3.Dignam, J., R. Lebovitz and R. Roeder. 1983. Accurate transcription initiation by RNA polymerase II ...
متن کاملDominant positive and negative selection using luciferase, green fluorescent protein and beta-galactosidase reporter gene fusions.
The introduction of exogenous DNA sequences into cultured cells is a cornerstone in the study of mammalian gene expression. For most cell types, the efficiency of stable gene transfer is usually quite low, which mandates the use of selectable markers to isolate a population of transfected cells with the desired phenotype. In simple gene expression experiments, only one type of selectable marker...
متن کاملDigital fluorescent pH sensors.
We designed polymeric sensors that created a digital-type fluorescence response to pH variation in an aqueous solution.
متن کاملSelective dendritic fluorescent sensors for Zn(II).
A series of dendritic 8-hydroxyquinoline (8-HQ) and 5-dialkyl(aryl)aminomethyl-8-HQ derivatives were synthesized and their fluoroionophoric properties toward representative alkali, alkaline earth, group IIIA and transition metal ions were investigated. Among the selected ions, Zn(II) enhanced the fluorescence of N-di-(methoxycarbonylethyl)aminoethyl-3-[4-(8-hydroxyquinolin-5-ylmethyl)piperazin-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: SCIENTIA SINICA Chimica
سال: 2017
ISSN: 1674-7224
DOI: 10.1360/n032017-00030